The single wavelength LED is powered by an LED driver, and then the illumination output is fiber-coupled into a patch cable, which connects to the implanted cannula. The most straightforward method for in vivo light stimulation of a specimen is to use a single fiber optic with a single LED light source. Single-Site Stimulation One Light Source to One Cannula Implant Please contact Technical Support for assistance with items outside the scope of this guide, including custom fiber components for optogenetics. Thorlabs offers a wide range of optogenetics components the compatibility of these products in select standard configurations is discussed in detail here. Two Light Sources into One Dual-Core Cannula Implant One or Two Light Sources to Two Cannula Implants

One Light Source to Two Cannula Implants Using Rotary Joint Splitter

The patch cable can be released by loosening the setscrew with the included hex key. To disconnect the interconnect from a cannula, squeeze the interconnect and gently pull the interconnect until disconnected from the cannula.

When using the dual core cannula and patch cable, please note the orientation of the patch cable for alignment purposes.ĭisconnecting the Cannula and Patch Cable.

If the cannula is already implanted or glued into position, adjust the cable-side ferrule, if necessary, to ensure physical contact between the ferrules.

Check that the ferrule tip of the cannula is clean and free of dust.

To lock the cannula in place, simply let go of the interconnect.

To connect to a cannula, gently squeeze the interconnect from both sides where indicated by the engraving, then insert the cannula ferrule into the open end until physical contact with the patch cable ferrule is achieved. For best results, the patch cable should be inserted past the setscrew and about halfway into the ceramic mating component within the interconnect.Ensure that the patch cable ferrule tip is clean and free of dust we recommend our canned compressed air.To secure the patch cable, use the included hex key to tighten the setscrew onto the inserted ferrule. Insert the patch cable ferrule into the interconnect on the side with the stainless steel setscrew. This tab contains instructions for using an interconnect to mate an optogenetics patch cable with a Ø1.25 mm or Ø2.5 mm ferrule to a cannula of the same diameter. Please see the animation below for an illustration of the installation process detailed here.

Stainless steel 6.4 patch